![]() ![]() Sequence logos have been widely used as graphical representations of conserved nucleic acid and protein motifs. The significance level was set at 0.05 for all tests. For yeast NatA substrate specificity analyses, the same set of subsequences of 25 residues from the N-termini of the 285 NatA substrates was used as the input set, while background models for Fisher’s exact test and Z-test were built from all subsequences and from randomly sampled subsequences of 25 AA residues from the N-termini of the yeast proteins not including the 285 NatA substrates, respectively. For human GRB substrate specificity analyses, the same set of 416, equal-length subsequences of 30 residues centered on the cleavage sites was used as the input set, while background models for Fisher’s exact test and Z-test were built from all subsequences and from randomly sampled subsequences of 30 AA residues from the UniProt human reference proteome, respectively. Fisher’s exact test ( A and C) and Z-test ( B and D) were performed to identify substrate sequence preferences of human GRB and yeast NatA, with a significance level of 0.05. S3 Fig: dagLogos resulting from different statistical test methods. ![]()
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